Thrombolytic, Analgesic and Antidiarrhoeal Activities of Methanol Extract of Bauhinia Acuminata Linn

Abstract

The present study was designed to evaluate the analgesic and antidiarrheal activities of a methanol extract and thrombolytic activity of methanol extract and its different partitionates obtained from the leaves of Bauhinia acuminata Linn. The analgesic activity was determined on mice model for its central and peripheral pharmacological actions using acetic acid-induced writhing and tail immersion tests, respectively and antidiarrheal effect was assessed by castor oil-induced diarrhea model at 200 and 400 mg/kg b.w. In in vitro thrombolytic study, the petroleum ether soluble materials of methanol extract revealed highest thrombolytic activity with clot lysis value of 46.66±0.67% as compared to 63.26±0.47% exhibited by the standard streptokinase. The extract significantly (P< 0.05) attenuated the acetic acid-induced writhing with the highest activity observed at 400 mg/kg b.w. (41.43%) comparable to that of the standard drug, diclofenac sodium (47.62%). A significant dose-dependent increase (P< 0.05) of latency period was also observed in the tail immersion method. During the castor oil-induced diarrheal assay, the extract showed significant (P<0.05) and dose dependant antidiarrheal effect. These findings indicate that sthe extract has potential thrombolytic, analgesic and antidiarrheal activity which support the folkloric claim and thus have great potential as a source of natural products.